E-ISSN 2146-8133
 

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Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo.

Cited by (1)

Abstract
Cell-based therapy is a promising treatment for metabolic disorders such as type-1 diabetes. Transplantation protocols have investigated several anatomical sites for cell implantation; however, some of these procedures, such as intraportal infusion, can cause organ failure or thrombosis secondarily. Bio-artificial organs could be the choice, although concerns still remain. Using a subcutaneous device, we are able to preserve neonatal porcine islets without sertoli cells in healthy New Zealand rabbits. Devices were implanted in the back of the animals underneath the skin, and after 3 months the islets were transplanted. Histology showed the presence of inflammatory cells, predominantly eosinophils; however, insulin- and glucagon-positive cell clusters were identified inside the device at different time points for at least 90 days, and porcine C-peptide was also detected during the follow-up, indicating graft functionality. We have found that our device induces the deposition of a fibrous matrix enriched in blood vessels, which forms a good place for cell grafting, and this model is probably able to induce an immunoprivileged site. Under these conditions, transplanted porcine islet cells have the capability of producing insulin and glucagon for at least three months.

Key words: Bio-artificial organs, cell-based therapy, diabetes, islets of Langerhans, xenotransplantation


 
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How to Cite this Article
Pubmed Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo. Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits. Arch Clin Exp Surg. 2013; 2(2): 101-108. doi:10.5455/aces.20121118030048


Web Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo. Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits. http://www.ejmaces.com/?mno=25440 [Access: November 14, 2018]. doi:10.5455/aces.20121118030048


AMA (American Medical Association) Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo. Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits. Arch Clin Exp Surg. 2013; 2(2): 101-108. doi:10.5455/aces.20121118030048



Vancouver/ICMJE Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo. Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits. Arch Clin Exp Surg. (2013), [cited November 14, 2018]; 2(2): 101-108. doi:10.5455/aces.20121118030048



Harvard Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo (2013) Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits. Arch Clin Exp Surg, 2 (2), 101-108. doi:10.5455/aces.20121118030048



Turabian Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo. 2013. Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits. Archives of Clinical and Experimental Surgery (ACES), 2 (2), 101-108. doi:10.5455/aces.20121118030048



Chicago Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo. "Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits." Archives of Clinical and Experimental Surgery (ACES) 2 (2013), 101-108. doi:10.5455/aces.20121118030048



MLA (The Modern Language Association) Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo. "Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits." Archives of Clinical and Experimental Surgery (ACES) 2.2 (2013), 101-108. Print. doi:10.5455/aces.20121118030048



APA (American Psychological Association) Style

Rafael Valdés-González, Ana L. Rodriguez-Ventura, Briceyda González-Ramírez, Benjamín León-Mancilla, Pedro Valencia, María del Carmen García de León, Ruy Pérez-Tamayo (2013) Long-Term Survival of Neonatal Porcine Islets Without Sertoli Cells in Rabbits. Archives of Clinical and Experimental Surgery (ACES), 2 (2), 101-108. doi:10.5455/aces.20121118030048





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